Separate Saccharification and Fermentation for Bioethanol Production from Raw Seaweed Sargassum sp.
The purpose of this study to obtain the best reducing sugars using acid solvent H2SO4 and HCl to be used as a substrate in fermentation processes to produce bioethanol. The research phase includes the preparation of raw materials Sargassum sp., the processing of acid hydrolysis used a solvent H2SO4 and HCl. Hydrolysis then fermented for five days for the production of etanol. Hydrolysis using acid solvent H2SO4 obtained the best acid concentration of 2% with the result of reducing sugars 82,62 g/L, whereas using HCl acid solvent obtained the best acid concentration of 2% with the result of reducing sugars 74,79 g/L. Fermented for 120 hours to produce ethanol each H2SO4 2 ml and 3 ml HCl.