ISOLASI DAN KARAKTERISASI BAKTERI PROTEOLITIK ASAL TERASI IKAN SELUANG (Stolephorus sp.)

Authors

  • Yulia Oktavia Program Studi Teknologi Hasil Perikanan, Fakultas Ilmu Kelautan dan Perikanan, Universitas Maritim Raja Ali Haji, Tanjungpinang, 29115, Indonesia
  • Shanti Dwita Lestari Program Studi Teknologi Hasil Perikanan, Fakultas Pertanian, Universitas Sriwijaya, Indralaya, 30862, Indonesia
  • Susi Lestari Program Studi Teknologi Hasil Perikanan, Fakultas Pertanian, Universitas Sriwijaya, Indralaya, 30862, Indonesia
  • Ace Baehaki Program Studi Teknologi Hasil Perikanan, Fakultas Pertanian, Universitas Sriwijaya, Indralaya, 30862, Indonesia
  • Dwiky Satya Kusuma Program Studi Teknologi Hasil Perikanan, Fakultas Pertanian, Universitas Sriwijaya, Indralaya, 30862, Indonesia

DOI:

https://doi.org/10.31629/marinade.v5i02.4943

Keywords:

anchovy, fermentation, proteolytic, Stolephorus sp.

Abstract

Anchovy is one type of processed fishery product in Indonesia that is made through a fermentation process. The fish paste fermentation process utilizes the activity of hydrolytic bacteria present in the raw materials. These bacteria can produce hydrolytic enzymes capable of hydrolyzing complex molecules into simple ones. This study aims to isolate proteolytic bacteria and determine the character of the bacterial isolates obtained. The test results were analyzed descriptively. The test parameters carried out were determining the proteolytic index, colony and cell morphology tests, and biochemical tests.  4 bacterial isolates had proteolytic activity, namely PT1, PT2, PT3, and PT5. Based on biochemical tests, the PT2 isolate was suspected to be proteolytic lactic acid bacteria.

 

 

Downloads

Download data is not yet available.

References

Alemu F. 2015. Isolation and screening of protease enzyme producing bacteria from cheese at Dilla University, Ethiopia. International Journal of Nutrition and Food Sciences. 4(2):234-239.
Amelia R., Philip K., Pratama Y.E., Purwati E. 2021.Characterization and probiotic potential of lactic acid bacteria isolated from dadiah sampled in West Sumatra Rinita Food Science and Technology. 41(2): 746-752.
Damayanti S.S., Komala O., Effendi E.M. 2018. Identifikasi bakteri dari pupuk organik cair isi rumen sapi. Ekologia : Jurnal Ilmiah Imu Dasar dan Lingkungan Hidup. 18(2):63-71.

Fadli R. A., Ibrahim M. N., Sadimantara S. 2016. Analisis kandungan zat pengawet pada terasi yang diperdagangkan di pasar tradisional Kota Kendari. J. Sains dan Teknologi Pangan. 1(1):73-78.
Fallo G. dan Sine Y. 2016. Isolasi dan uji biokimia bakteri selulolitik asal saluran pencernaan rayap pekerja (Macrotermes spp.) Jurnal Pendidikan Biologi. 1(2):27-29.
Finanda A., Mukarlina, Rahmawati. 2021. Isolsi dan karakterisasi genus bakteri asam laktat dari fermentasi daging buah pisang kepok (Musa paradisiaca L.). Protobiont. 10 (2):37-41.
Kosasi C., Lolo W. A., Sedewi S. 2019. Isolasi dan uji aktivitas antibakteri dari bakteri yang berasosiasi dengan alga Turbinaria ornata (Turner) J. Agardh serta Identifikasi secara biokimia. Pharmacon. 8(2): 351-359.
Kurahman O.T., Yuliawati A., Haerunnisa L., Supriyatna A., Cahyanto T., Suryani Y., Supriadin A., Hidayat C., Masri M. 2020. The isolation and identification bacteria on jallalah animal (study on the feeding tilapia (Oreochromis niloticus) with chicken manure as foods. Elkawnie: Journal of Islamic Science and Technology. 6(2):222-236.
Laily I.N., Utami R., Widowati E. 2013. Isolasi dan karakterisasi bakteri asam laktat penghasil riboflavin dari produk fermentasi sawi asin. Jurnal Aplikasi Teknologi Pangan, 2(4):179-184.
Maflahah I. 2013. Kajian potensi pembuatan terasi udang studi kasus di Desa Bantelan, Kecamatan Batu Putih, Kabupaten Sumenep. Fakultas Pertanian Universitas Trunojoyo. Madura.
Manalu R.T., Febriani A., Syamsinar. 2021. Isolation and characterization of lactic acid bacteria in fermented sweet corn (Zea mays L.) as antibacterial. Bioscience. 5(2): 141-149.
Misgiyarta, Widowati S. 2013. Seleksi dan karakterisasi bakteri asam laktat (BAL) indigenus. Prosiding Seminar Hasil Penelitian Rintisan dan Bioteknologi Tanaman.377-387.
Puspitasari F.D., Shovitri M., Kuswytasari N. D. 2012. Isolasi dan karakterisasi bakteri aerob proteolitik dari tangki septik. Jurnal Sains dan Seni ITS. 1(1): E1-E4.
Putri I., Jannah S.N., Purwantisari S. 2020. Isolation and characterization of lactic acid bacteria from Apis mellifera and their potential as antibacterial using in vitro test against growth of Listeria monocytogenes and Escherichia coli. NICHE Journal of Tropical Biology. 3(1):26-34.
Sharma A.K., Sharma V., Saxena J., Yadav B., Alam A., Prakash A. 2015. Optimization of protease production from bacteria isolated from soil. Applied Research Journal. 1(7): 388-394.

Downloads

Published

2022-10-31

Issue

Section

Articles