a- ENZYME XYLANASE ACTIVITY TEST USE DNS METHOD TO KNOWN HIGH ACTIVITIES OF ENZYME XYLANASE

Activity for absorb hemicellulose

Abstract

Xylanase is extracellular enzim which can hydrolyze Xylan (hemicellulose) become  Xylo-oligosaccharides and Xylose. DNS is reagent that can be use to     calculate the quantitative assay of glucose. This reagent is function to give colour to the solution, so that can read by spectrofotometes vis which read the colour from the solution. Quantitative analysis of glucose with Dynitrosalicylic  Acid (DNS) method. The reaction with DNS  occur  are  Oxydation and reduction reaction in  aldehyde form of glucose and oxidized become carbocsyl fotm. Meanwhile the DNS as  oxidizer will reduced and form 3-amyno and 5-nitrosalicylic acid. The reaction  goes by  alkaline condition. When there  are reducing sugar on the sample, so the DNS solution which was initially yellow will reacting with reducing sugar that causing the DNS solution’s colour become  reddish orange.