IDENTIFIKASI SENYAWA BIOAKTIF PADA KULIT IKAN SEMBILANG (Paraplotosus albilabris) DAN POTENSINYA SEBAGAI ANTIBAKTERI

Penulis

  • Yulia Oktavia Program Studi Teknologi Hasil Perikanan, Fakultas Ilmu Kelautan dan Perikanan, Universitas Maritim Raja Ali Haji, Tanjungpinang, 29111, Indonesia
  • Made Suhandana Dinas Kelautan dan Perikanan Provinsi Sumatera Selatan, Palembang, 30252, Indonesia
  • Alen Fri Jacky Pasaribu Alumni Program Studi Teknologi Hasil Perikanan, Fakultas Ilmu Kelautan dan Perikanan, Universitas Maritim Raja Ali Haji, Tanjungpinang, 29111, Indonesia

DOI:

https://doi.org/10.31629/marinade.v6i01.5027

Kata Kunci:

antibakteri, komponen bioaktif, ikan sembilang, toksisitas

Abstrak

Ikan sembilang adalah salah satu biota perairan yang mengandung senyawa bioaktif. Senyawa bioaktif ini yang memiliki potensi untuk dikembangkan menjadi antikanker, antimikroba, dan antioksidan. Tujuan penelitian ini yaitu untuk mengekstraksi senyawa aktif dan menguji potensinya sebagai anti bakteri. Maserasi dengan menggunakan tiga jenis pelarut organik yang berbeda (etanol, metanol, n heksan) digunakan untuk mengekstrak komponen bioaktif pada kulit ikan sembilang. Parameter uji yang dilakukan yaitu identifikasi komponen bioaktif, uji toksisitas (Brine Sirmp Lethaly Test), dan uji antibakteri (agar difusi). Hasil penelitian ini menunjukkan bahwa pelarut etanol dan metanol mampu mengekstrak lebih banyak komponen bioaktif daripada pelarut n-heksan, nilai LC50 ekstrak kulit ikan sembilang (etanol, metanol, dan n-heksan) yaitu 451,42 ppm; 33853,22 ppm; 29513,84 ppm. Hasil ekstraksi dengan pelarut etanol memiliki zona hambat tertinggi yaitu 93 mm untuk bakteri Staphylococcus aureus dan 74 mm untuk bakteri Escherichia coli. Diduga bioaktif tersebut tergolong senyawa bioaktif polar.

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2023-04-02

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Vol 6 No 01 (2023): Marinade

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Cara Mengutip

Oktavia, Y., Suhandana, M., & Pasaribu, A. F. J. (2023). IDENTIFIKASI SENYAWA BIOAKTIF PADA KULIT IKAN SEMBILANG (Paraplotosus albilabris) DAN POTENSINYA SEBAGAI ANTIBAKTERI. Marinade, 6(01), 26-33. https://doi.org/10.31629/marinade.v6i01.5027