Effectiveness of Nitrogen Sources in Different Organic Fertilizers as Urea Substitutions in Nutrient Culture of Nannochloropsis Sp.

Authors

  • Paulin Sinurat Universitas Maritim Raja Ali Haji
  • Henky Irawan Universitas Maritim Raja Ali Haji
  • Dwi Septiani Putri Universitas Maritim Raja Ali Haji

Keywords:

Nannochloropsis sp., Urea Substitution, Nitrogen, Organic Fertilizer

Abstract

Nitrogen is one of the nutrients needed Nannochloropsis sp., but there is no determination of fertilizer dosage based on fertilizer nitrogen source. This study aims to determine the provision of organic fertilizers capable of substituting urea in nutrient cultures Nannochloropsis sp. and effective used as a source of nitrogen for cell populations Nannochloropsis sp.. This research was conducted in April-May 2025 at the Batam Marine Aquaculture Center. This study used a complete randomized design (RAL) with 1 Control and 3 treatments, namely: control (50 mg/L Urea, Za, TSP, EDTA), treatment a (dung bats, Za, TSP, EDTA), treatment B (Quail Dung, ZA, TSP, EDTA), treatment C (Dung swallows, ZA, TSP, EDTA), each 3 replications. The observed parameters include daily density, population peak, doubling time, light intensity and water quality. Results showed for 7 days maintenance of cell density Nannochloropsis sp. the highest growth was found in h-5 maintenance with the highest growth in treatment B (quail droppings) is 23.193.055 cells/ml with the fastest doubling time is 36.3 hours and the lowest growth in control is 17.468.056 cells/ml with the longest doubling time is 52.3 hours. The peak population for control and all treatments is found on H-5 maintenance. The light intensity during the study ranged from 9.100 lux. This result shows that the fertilizer of bat droppings, quail droppings and bird droppings can be a substitution of urea and effective as a source of nitrogen in the nutrient culture Nannochloropsis sp. for cell populations Nannochloropsis sp..

Downloads

Download data is not yet available.

References

Anjellyta, D. (2023). Teknik Kultur Nannochloropsis sp. Menggunakan Pupuk Teknis Skala Semi Massal di Balai Perikanan Budidaya Laut (BPBL) Batam.

daefuArfah, Y., Cokrowati, N., & Mukhlis, A. (2019). Pengaruh Konsentrasi Pupuk Urea terhadap Pertumbuhan Populasi Sel Nannochloropsis sp. Jurnal Kelautan, 12(1), 45. https://doi.org/10.21107/jk.v12i1.4925

BIOMLaboratoryPractical-UQ. (2021). Counting Cells on a Haemocytometer. https://www.youtube.com/watch?v=MZV4_QkonAE

Blank, L. M. (2012). The Cell and P: from Cellular Function to Biotechnological Application. Current Opinion in Biotechnology, 23(6), 846–851. https://doi.org/10.1016/J.COPBIO.2012.08.002

Daefi, T., Tugiyono, Rusyani, E., & Murwani, S. (2017). Pertumbuhan dan Kandungan Gizi Nannochloropsis sp. yang Diisolasi dari Lampung Mangrove Center dengan Pemberian Dosis Urea Berbeda pada Kultur Skala Laboratorium. Jurnal Biologi Eksperimen Dan Keanekaragaman Hayati, 4(1), 39–46. https://doi.org/https://doi.org/10.23960/j-bekh.v4i1.2186

Damanik, R., Komariyah, S., & Putriningtias, A. (2020). Pengaruh Penggunaan Warna Cahaya yang Berbeda terhadap Pertumbuhan Nanochloropsis sp. Journal of Aquaculture Science, 5(September), 99–109. https://doi.org/https://doi.org/10.31093/joas.v5i2.111

Ernest, P. (2012). Pengaruh Kandungan Ion Nitrat terhadap Pertumbuhan Nannochloropsis sp. Universitas Indonesia.

Fatmanita, Nurrachmi, I., & Feliatra, F. (2023). Efektivitas Kandungan Nitrogen dalam Pupuk Berbeda terhadap Klorofil pada Nannochloropsis sp. Skala Laboratorium. Jurnal Ilmu Perairan (Aquatic Science), 11(3), 193–201. https://doi.org/http://dx.doi.org/10.31258/jipas.11.3.p.192-201

Fauzan, M., Siregar, S. H., & Nasution, S. (2021). Effect of Different Types of Fertilizer on the Growth of Marine Phytoplankton Population Chlorella vulgaris. 4(1), 65–72.

Geider, R. J., & La Roche, J. (2002). Redfield Revisited: Variability of C:N:P in Marine Microalgae and Its Biochemical Basis. European Journal of Phycology, 37(1), 1–17. https://doi.org/10.1017/S0967026201003456

Jadid, R., Dewiyanti, I., & Nurfadillah. (2017). Penambahan Air Kelapa pada Media Pertumbuhan Populasi Nannochloropsis sp. Jurnal Ilmiah Mahasiswa Kelautan Dan Perikanan Unsyiah, 2(1), 113–118. https://media.neliti.com/media/publications/187053-ID-penambahan-air-kelapa-pada-media-pertumb.pdf

Khuril’ain, W. (2017). Pengaruh Indole Acetic Acid (IAA) terhadap Pertumbuhan dan Kadar Lipida Nannochloropsis oculata. [UIN Sunan Gunung Djati Bandung]. https://digilib.uinsgd.ac.id/6791/

Lin, S., Litaker, R. W., & Sunda, W. G. (2016). Phosphorus Physiological Ecology and Molecular Mechanisms in Marine Phytoplankton. Journal of Phycology, 52(1), 10–36. https://doi.org/10.1111/jpy.12365

Manisali, A. Y., Sunol, A. K., & Philippidis, G. P. (2019). Effect of Macronutrients on Phospholipid Production by the Microalga Nannochloropsis oculata in A Photobioreactor. Algal Research, 41, 101514. https://doi.org/10.1016/J.ALGAL.2019.101514

Putri, S. A., & Sopandi, T. (2021). Konsumsi Nitrogen dan Karbon Oleh Spirulina platensis dari Kotoran Burung Puyuh sebagai Media Kultivasi. Stigma, 14(1), 1–9. https://doi.org/https://doi.org/10.36456/stigma.14.01.3492.1-9

Rizki, M. (2023). Pengaruh Pemberian Pupuk Organik Cair (POC) Biourin Kambing terhadap Kepadatan Sel dan Laju Pertumbuhan Nannochloropsis sp. [Universitas Malikussaleh]. https://rama.unimal.ac.id/id/eprint/577/5/Muhammad Rizki_190330013_Pengaruh Pemberian Pupuk Organik Cair %28POC%29.pdf

Ryu, R., Rohyani, I. S., & Ali, L. (2015). Pertumbuhan Tetraselmis dan Nannochloropsis pada Skala Laboratorium (Vol. 1, Issue 5, pp. 296–299). https://doi.org/10.13057/psnmbi/m010221

Sartika, E., Siswoyo, B. H., & Syafitri, E. (2021). Pengaruh Pakan Alami yang Berbeda Terhadap Pertumbuhan dan Kelangsungan Hidup Benih Ikan Mas Koi (Cyprinus rubrofuscus). Jurnal Aquaculture Indonesia, 1(1), 28–37. https://doi.org/10.46576/jai.v1i1.1437

Setiyaningrum, L. (2012). Penggunaan Pupuk Anorganik (UREA, SP 36 DAN NPK) terhadap Laju Pertumbuhan Populasi Nannochloropsis sp. Universitas Brawijaya.

Sinurat, P. B. (2025). Teknik Kultur Nannochloropsis sp. Skala Massal di Balai Perikanan Budidaya Laut (BPBL) Batam.

Solovchenko, A. E., Ismagulova, T. T., Lukyanov, A. A., Vasilieva, S. G., Konyukhov, I. V, Pogosyan, S. I., Lobakova, E. S., & Gorelova, O. A. (2019). Luxury Phosphorus Uptake in Microalgae. Journal of Applied Phycology, 31(5), 2755–2770. https://doi.org/10.1007/s10811-019-01831-8

Syofiani, R., & Oktabriana, G. (2017). Aplikasi Pupuk Guano dalam Meningkatkan Unsur Hara N, P, K, dan Pertumbuhan Tanaman Kedelai pada Media Tanam Tailing Tambang Emas. Prosiding Seminar Nasional 2017 Fakultas Pertanian UMJ, 98–103. https://jurnal.umj.ac.id/index.php/semnastan/article/view/2264

Talino, H., Zulfita, D., & Surachman. (2013). Pengaruh Pupuk Kotoran Burung Walet terhadap Pertumbuhan dan Hasil Tanaman Kacang Hijau pada Tanah Aluvial. Jurnal Sains Pertanian Equator, 2(2). https://doi.org/10.26418/jspe.v2i2.2476

Utami, N. P., MS, Y., & Haetami, K. (2012). Pertumbuhan Chlorella sp. yang Dikultur pada Perioditas Cahaya yang Berbeda. Jurnal Perikanan Dan Kelautan, 3(3), 237–244.

Viqram, V., Abidin, Z., & Mukhlis, A. (2018). Pengaruh Penambahan Pupuk Organik Guano dengan Konsentrasi yang Berbeda terhadap Laju Pertumbuhan Spirulina sp. Jurnal Perikanan Unram, 8(2), 58–65. https://doi.org/10.29303/jp.v8i2.119

Additional Files

Published

2026-04-30

How to Cite

Effectiveness of Nitrogen Sources in Different Organic Fertilizers as Urea Substitutions in Nutrient Culture of Nannochloropsis Sp. (2026). Jurnal Intek Akuakultur, 8(1), 22-30. https://ojs.umrah.ac.id/index.php/intek/article/view/7478